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Applied Biosystems® 7500 Fast Real-Time PCR System

Catalog number: 4351106

Description:

The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes. We supply a Dell™ Notebook computer with the 7500 Fast System.

  • Specially designed Fast block ensures thermal uniformity at top speeds.
  • Faster ramp rates enable rapid results without compromising extension times or assay quality.
  • Fast optical plates ensure excellent precision in 10-30 ul reaction volumes.
  • Use TaqMan® Fast Universal PCR Master Mix to run multiple Fast assays on one plate.
  • Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System.

High Resolution Melting Analysis
The Applied Biosystems® High Resolution Melting (HRM) Software is the easiest to use melting analysis software enabling real-time PCR melt curve assays to be used more accurately for mutation scanning and genotyping. The HRM Software is available on the 7500 Fast Real-Time PCR System, which delivers precise results in a standard 96-well format.

Use the Applied Biosystems® High Resolution Melting (HRM) Software to perform more sophisticated melting analysis with an easy to follow workflow and minimal subjective data analysis steps.

  • Shortens analysis time by auto-calling genotypes and automatically omitting the no template controls.
  • Minimizes subjective analysis by automatically grouping unknown variant clusters.
  • No temperature shift required – distinguish between homozygous mutants and wild type more easily.
  • Allows easy data review with customizable multi-plot views, expandable windows and one-click color assignment to highlight curves of interest.
  • Facilitates data presentation with the option to export data and graphs directly to PowerPoint or as JPEG files.
  • 21 CFR Part 11 Module available
  • The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
  • Individual user log-ins can be added for up to four user groups, each group with designated permission settings.
  • User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts.
  • Audit trails can be enabled or disabled depending on your traceability needs.
  • A selection of e-signatures is available to ease e-signatures into your workflow.


One System, Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 Fast system. Now you can reduce your assay optimization efforts.

Specifications:

Calibrated Dye:

Cy3, Cy5, FAM, JOE, NED, ROX, SYBR Green, TAMRA, Texas Red, VIC

Capacity:

1 x 96-well plate (Fast), 12 x 8-tube strips

Dimensions:

34 cm (13.39 in) (W) x 49 cm (19.29 in) (H) x 45 cm (17.72 in) (D)

Dynamic Range:

9 logs of linear dynamic range

External Computer:

Laptop computer provided

For Use With (Equipment):

7500 Fast System

Format:

8-well strips, 96-well plate

Optics:

CCD Camera, Five excitation and five emission filters, Halogen light source

Passive Reference Dye:

Other Passive Reference Dye, ROX (Pre-mixed), ROX (Separate Tube)

Peak Block Ramp Rate:

5.5°C ⁄ sec

Precision:

Distinguish between 5,000–10,000 genome equivalents (two-fold copy number difference) with 99.7% confidence

Product Size:

1 system

Reaction Speed:

Fast, Standard

Reaction Volume Range:

10-30 µl

Run Time:

<2 hrs (Standard), 36 min (Fast)

Sample Ramp Rate:

Fast mode: ± 3.5°C ⁄ sec, Standard mode: ± 1.6°C⁄sec

Sensitivity:

Down to 1 copy of human RNAse P gene

Temperature Accuracy:

±0.5°C

Temperature Range (Metric):

4.0-99.9 °C

Temperature Uniformity:

±1°C

Thermal Cycling System:

Peltier-Based System

Throughput:

4 to 5 96-well plates per day

Weight:

34 kg (75 lb)